recently I have had much success stopping pesky proteins sticking to
my SEC columns by sticking 10% galactose in my buffer.

cheers
andy

2009/4/16 conancao <conan_...@hotmail.com>:
> Hi:
>
>    I have seen some protocols(IMPACT from New England Lab etc.) which can
> overexpress protein with a tag then cut or displace the tag off to get a
> native protein. Maybe those are the options.
>
> Best,
> Hongnan Cao
> UCR
>
>
>
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