recently I have had much success stopping pesky proteins sticking to my SEC columns by sticking 10% galactose in my buffer.
cheers andy 2009/4/16 conancao <conan_...@hotmail.com>: > Hi: > > I have seen some protocols(IMPACT from New England Lab etc.) which can > overexpress protein with a tag then cut or displace the tag off to get a > native protein. Maybe those are the options. > > Best, > Hongnan Cao > UCR > > > > ________________________________ > 立刻下载 MSN 保护盾,保障Messenger 安全稳定! 现在就下载!