Dear All, I have a crystal growing in the presence of 0.1M Sodium Acetate pH 5.0, 10% PEG4000, 7.5% Dioxane and 10% Ethylene Glycol. I wanted to know if it would be alright to use Samarium chloride for derivatization. I am worried about the leaching of samarium by acetate. Also, what soak times can I use and what conc. of Samarium chloride? My protein is 10 kDa , has no cysteines/methionines and pI for the molecule is 4.6. Also, could somebody suggest what other heavy atom soaks could be performed in this case?
Any suggestions would be appreciated. Many thanks in advance -- Amit Sharma
