[ccp4bb] Link two proteins into one polypeptide

[Raji Edayathumangalam]

Hi People,

Could anyone point me to successful examples for two unrelated  
proteins that have been stitched together into one single polypeptide  
chain with flexible amino acids to create a functional chimera that  
was subsequently crystallized. I've looked up a few.

I am particularly interested in understanding all the important  
considerations while designing a flexible linker even though many of  
these factors might be case dependent might be variable, obvious and  
commonsensical. Either way, I'd like to hear what folks have to say.

Thanks very much.
Raji