One can estimate this from http://www.ruppweb.org/new_comp/anomalous_scattering.htm
and the answer as Jim says is no. BR -----Original Message----- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk] On Behalf Of Jim Pflugrath Sent: Friday, March 20, 2009 1:08 PM To: CCP4BB@JISCMAIL.AC.UK Subject: Re: [ccp4bb] Problems with phasing a protein (1300aa) Well, what do you expect the anomalous signal contributed from your 45 seleniums in a perfect world to be when the asymmetric unit contains 1300 aa? Do you think a dataset with Rmerge of ~15% is good enough to detect a signal of even 2%? (Note: I did not do the calculation, so I just made up the number of 2%.) Jim On Fri, 20 Mar 2009, Kumar wrote: > Hello CCP4bb members, > > I have been trying to obtain phases for a protein which contain ~1300aa. We > have obtained native data to a resolution of 3.3A (Space group I222 or > I212121). But we are having tough time phasing it. >...
