Hi, Sorry for the off topic question. I am looking for helps from someone who is familiar with Centramate from Pall. We recently bought one and try to use it for concentrating insect media with secreted proteins. Here is some more information: Pump: MasterFlex Console drive Pump head: quick load, model 7021-26 Tubing: We originally use PharMed L/S24. However, we quickly realized that the pump head will cut the tube. We then switched to PharMed L/S15. Membrane: We use an Omega cassette with 10k cutoff. Our protein is about 25 kDa. We use Hyclone SFX-insect media. The supernatant is pre-filtered using a 0.45 um membrane before being loaded to Centramate. We add a pressure at about 20 psi with about 8 psi backpressure. It is quite fast at the beginning and then turns slower and slower. For the experiment I just did today, it took about 5 hours to go from 2L down to about 300 ml. This is ridiculous! Did I do something wrong? The tech at Pall insisted that it must be due to some strange ingredient in the Hyclone media. Anyone agree? Any common will be highly appreciated. Best, Chen
