hi folks i've almost got my B-factors questions sorted out. one thing i still don't understand is this:
in truncate's wilson plot, what is plotted on the vertical axis is not ln I_mean, but ln (I_mean/ff_mean) and ff_mean (or Mn_ff, as truncate calls it) is the "average expected value of ff". my question is how is this value calculated? its resolution dependence obviously affects the B-factor, so i would like to understand at least what goes into it. at this point in the analysis, all truncate knows is the cell/spacegroup, and the approximate number of AAs right? is this just an expectation of how the amplitudes ought to fall off vs spacial frequency, without knowing anything about the detailed structure? thanks!
