Yingjie Peng wrote:
..
After I have solved my strucutre, I have found my target ligand bound
at the potential binding site. Also, I have
found that there are two more ligand molecules bound along the path
from solvent to the binding site. I think this
can enrich the ligand to binding site, enhancing the local
concentration of the ligand, thus reducing the Km of the
ligand.
I'm not sure if this is anything like what you see, but sometimes more
than one ligand molecule unexpectedly binds in the active site. e.g.
http://www.rcsb.org/pdb/explore.do?structureId=1GX
http://www.ncbi.nlm.nih.gov/pubmed/15102853?dopt=Abstract
Cheers,
Charlie
--
Charlie Bond
Professorial Fellow
University of Western Australia
School of Biomedical, Biomolecular and Chemical Sciences
M310
35 Stirling Highway
Crawley WA 6009
Australia
charles.b...@uwa.edu.au
+61 8 6488 4406
