Sure, it's not always 'disastrously bad' to have EDTA (hence my use of the word 'bad' rather than a more categorical statement. Donuts are bad for me yet I can't stop eating them :)
Yes, you can take a risk. However since periplasmic isolation is already a PITA, why add an extra concern? Artem > Artem, > > Artem Evdokimov wrote: >> Please note that osmotic shock extraction typically employs EDTA which >> is >> obviously bad for IMAC. >> > This is not entirely correct. > I have used extracts with 5 mM EDTA for IMAC in the past. > If your IMAC column volume is large enough, only the top 1-2 mm will be > depleted of Me2+ (easily seen with Cu2+). > Moreover, it is always possible to add some Me2+ to your extract prior > to IMAC. > All this was published long ago (Biochemistry. 31: 2690-2702, 1992). > > Nadir > > -- > > Pr. Nadir T. Mrabet > Cellular & Molecular Biochemistry > INSERM U-724 > Nancy University, School of Medicine > 9, Avenue de la Foret de Haye, BP 184 > 54505 Vandoeuvre-les-Nancy Cedex > France > Phone: +33 (0)3.83.68.32.73 > Fax: +33 (0)3.83.68.32.79 > E-mail: nadir.mra...@medecine.uhp-nancy.fr > > > >
