Hello All, can anyone recommend a nice, free software package for analyzing binding curves? The application I have in mind is fluorescence polarization studies between a FITC-peptide and a protein, for which I already seem to have good data.
Thanks in advance, Jacob Keller ******************************************* Jacob Pearson Keller Northwestern University Medical Scientist Training Program Dallos Laboratory F. Searle 1-240 2240 Campus Drive Evanston IL 60208 lab: 847.491.2438 cel: 773.608.9185 email: j-kell...@northwestern.edu ******************************************* ----- Original Message ----- From: Michael Colaneri To: CCP4BB@JISCMAIL.AC.UK Sent: Friday, February 06, 2009 4:26 PM Subject: [ccp4bb] Hi prep column from GE We have a hi prep SP column from GE. We try to load ~14 mg protein but all goes to FT. We lowered the pH and changed the buffer but no luck. We would appreciate all suggestions. Mike Colaneri
