On Feb 2, 2009, at 11:39, Clemens Vonrhein wrote:
Hi Tassos,
On Mon, Feb 02, 2009 at 11:13:28AM +0100, Anastassis Perrakis wrote:
To be more detailed I would:
0. Put the mol rep solution in ARP/wARP and let it run (in the
meantime
proceed to 1-3 if 0. fails ...)
^^^
You didn't tell us what point 1 is:
2. Do some density modification using the model phases (if you are
in ccp4
simplest is to use DM)
3. Use the DM phases and pretend this is an 'experimental' solution
in
ARP/wARP
... are you hiding some secret, magic procedure from us?
Indeed. Point 1. was the NCS averaging for the dimeric coiled coil.
Then I realized it might not be a great idea for the coiled coil,
deleted it, and did not renumber.
Oh well - its Monday morning. And I have still not recovered from my
enthusiasm on the latest
magic of autoSHARP ;-)
A.
Cheers
Clemens
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P please don't print this e-mail unless you really need to
Anastassis (Tassos) Perrakis, Principal Investigator / Staff Member
Department of Biochemistry (B8)
Netherlands Cancer Institute,
Dept. B8, 1066 CX Amsterdam, The Netherlands
Tel: +31 20 512 1951 Fax: +31 20 512 1954 Mobile / SMS: +31 6 28 597791
