I've used serial cloner (http://serialbasics.free.fr/
Serial_Cloner.html) but not ApE, which I hope to try now that I know
of it. One thing that serial cloner has going for it is a nice
assembly tool that makes construct design much easier. Otherwise it
could be a little less clunky in its design.
<editorial>
These days there are too many free alternatives for invitrogen to try
to pilfer scientists. But I guess many companies are beginning to
learn what a "broken business model" really means.
If invitrogen were smart, they'd give their redundant NTI software
away to whoever will take it and push to make it a convenient front-
end ordering system for their other services which actually do have
value beyond the configuration of bits on a hard-drive. (Hint: be like
google.)
</editorial>
James
On Jan 28, 2009, at 1:14 AM, Sebastiano Pasqualato wrote:
Hi Darren,
much much "easier" than VectorNTI is ApE (http://www.biology.utah.edu/jorgensen/wayned/ape/
), which is multi-platform and very easy to use for simple tasks.
Please, could you post a summary of the answers?
Thanks,
ciao
Sebastiano
On Jan 28, 2009, at 9:47 AM, Darren Hart wrote:
Hello,
After several years of offering the molecular biology software
VectorNTI
free to the academic community (their "open access program") and
building
up a huge user base, Invitrogen have suddenly announced that they
will no
longer renew these free licences and the existing ones will be left
to
expire within the year. There are heavy renewal fees for anyone
wishing to
continue use of this software.
Can anyone recommend decent alternative PC compatible alternatives?
Main
uses are construct and primer design, plus simple quick alignments,
sequence data analysis etc. The database structure for storing
sequences
was pretty useful also.
Ideally free, otherwise reasonably priced. I've seen CLCbio and
Geneious
have products, both free and paid. Any experience?
Thanks,
Darren
EMBL Grenoble
ps anyone using VNTI might consider a backup of their work by
exporting
files to .gb format. I don't know if a locked up (expired) version
permits
this and you will have no notice that it is about to expire.
--
Sebastiano Pasqualato, PhD
IFOM-IEO Campus
Dipartimento di Oncologia Sperimentale
Istituto Europeo di Oncologia
via Adamello, 16
20139 - Milano
Italy
tel +39 02 9437 5094
