Look into the 0.5 sq. m Millipore Pellicon ultrafiltration/
microfiltration devices (http://www.millipore.com/catalogue/module/
c558). They are moderately expensive, but the membranes are robust,
reusable and allow fast concentration steps. We have regularly used
them to concentrate cells (microfiltration mode) and/or media
(ultrafiltration mode) from 16 L insect cell cultures to volumes of
500 mL in ~1 hr. Given the speed, you could use it for buffer exchange.
Michael
****************************************************************
R. Michael Garavito, Ph.D.
Professor of Biochemistry & Molecular Biology
513 Biochemistry Bldg.
Michigan State University
East Lansing, MI 48824-1319
Office: (517) 355-9724 Lab: (517) 353-9125
FAX: (517) 353-9334 Email: garav...@msu.edu
****************************************************************
On Jan 4, 2009, at 8:34 PM, Michael Hothorn wrote:
Dear all,
sorry for the non-CCP4 question. I am expressing a protein in insect
cells that is targeted for secretion into the medium. After
expression, I want to concentrate the medium containing my protein
of interest (from 10 l to 0.3 L) and at the same time change the
buffer to something that is compatible with my first ion exchange
chromatography step. I would be interested to know what kind of
ultrafiltration devices can be used for this purpose, and whether
some of you could suggest a quick, reliable and efficient system
that we could purchase for our lab.
Thanks!
Michael
