Hi there, I have trouble with normal glycerol cryoprotectant. My protein crystals seems not stable in it. However, my mother liquor has extremely high concertration of Nonyl-maltoside (estimated ~20%, this membrane protein really needs that high detergent, sounds weird.8) So, I am wondering anyone with successful experience using detergents as cryoprotant can give some comments. If they works well, that means I can directly fish some crystals out of mother liquor without adding other cryos.
Thanks a lot. Deliang
