[ccp4bb] GST fusion-Expression problem

[Putcha, Balananda Dhurjati Kumar]

Dear CCP4ers,
 
I have a GST fusion protein (eukaryotic).  When I express it in E.coli BL21 
(DE3) or RIPL cells, however, I mostly get GST and very little fusion protein.  
I get very little "leaky expression".  I normally grow my cells at 37C in LB or 
2X YT and after induction at 20C for 16-18hrs.  I also have tried growing at 
37C for 2hrs (after induction) with little change.  Is there a trick to solving 
this problem?   Thank you for your thoughts
Kumar