You might want to try acetonitrile. annie
Annie Hassell Glaxo Smithkline 5 Moore Drive RTP, NC 27709 919/483-3228 919/483-0368 (FAX) [EMAIL PROTECTED] "Heng Chiat Tai" <[EMAIL PROTECTED]> Sent by: "CCP4 bulletin board" <CCP4BB@JISCMAIL.AC.UK> 16-Oct-2008 16:15 Please respond to "Heng Chiat Tai" <[EMAIL PROTECTED]> To CCP4BB@JISCMAIL.AC.UK cc Subject [ccp4bb] How to improve twinning crystal in protein crystallization Hi, I have a protein crystallized in 30% PEG8000/2000 mM NaCl/Tris pH 7.0. The crystal is not single but twinned long rod. I tried glycerol and dioxane, but it doesn't work. Try to change pH also same. Any suggestion? Any paper mention provide systematic strategy for solving this protein crystallization issue? Rgds, HCT See how Windows Mobile brings your life together?at home, work, or on the go. See Now