Another suggestion that comes to mind is crosslinking via Cys, are you
purifying under reducing conditions ? Or since you have inclusion
bodies they're in general not correctly folded hence expose
hydrophobic regions and can interact with other proteins, to avoid
unspecific interaction add >20% glycerol to your lysis buffer.
Jürgen
On 23 Sep 2008, at 00:51, Meg wrote:
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Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
Web: http://faculty.washington.edu/jbosch
