Oh, another thing you can try is simply increasing the drop volume,
especially large sitting drops. This will slow down equilibration,
and there will be proportionately more material
On Sep 9, 2008, at 11:23 PM, Vincenzo Carbone wrote:
Dear all,
I was wondering if anyone had some practical advice in regards to
increasing
the size of a crystal. Currently my enzyme forms these rather nice
cubic and
very sharp <0.1mm (in 25% peg 5000, 0.1M ammonium sulphate, 0.1M
Tris ph
7.0) crystals following optimisation of the pH and precipitant
concentrations of the initial screening condition and by playing with
various increasing levels of protein concentration 20-60mg/ml.
Has anyone had success with additive screens in increasing crystal
size and
what were they?
Any advice would be useful thanks
Vince
