Hello everyone: Sorry for the off-topic question. I am trying to separate by SDS-PAGE really big proteins (>500 kDa), and lower percentage (3-8%) acrylamide gels do not do the trick. Based on literature searches, acrylamide-agarose composite gels seem the way to go. Is anyone willing to share a protocol? I cannot get hold of any of the old journals where this was described at our rather limited library...
Thanks a lot! Cheers, Alex
