James Pauff a écrit :
Hello all,
I have a refined structure at 2.6 angstroms that at about 73% completeness at
this resolution. The I/sigma is about 2.0 at 2.6 angstroms, and the omit
density for my ligands is great contoured at 3.0sigma. My Rcryst is 19 or so
and the Rfree is 24.5 or so.
HOWEVER, my mean B value is 13.9, whereas my other 2 structures (at 2.2 and 2.3
angstroms, same protein, >95% completeness) have mean B values of 22+. Any
suggestions as to what is going on here? I'm having trouble explaining this.
Thank you,
Jim
Dear Jim,
probably you did not collect your data to the highest possible
resolution. Did you use an inhouse source? You would expect that a
crystal with an average temperature factor of 14 A2 would diffract to
1.6 A on a synchrotron source.
Regards
Wim
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Wim Burmeister
Professeur, Membre de l'Institut Universitaire de France
Unit of Virus Host Cell Interactions (UVHCI) UMR5233 UJF-EMBL-CNRS
6 rue Jules Horowitz
B.P. 181, F-38042 Grenoble Cedex 9 FRANCE
E-mail: [EMAIL PROTECTED]
Tel: +33 (0) 476 20 72 82 Fax: +33 (0) 476 20 94 00
http://www.uvhci.fr
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