Those colorful tags may be too big for crystallization purpose. You can try GFP or heme based tags. A few companies are promoting heme based red tags. Red color is not very intense. You'd be better off with UV or GFP. There are small molecule tags that links to Cys. But you'll spend a fortune to get enough to see. They are Ni-NTA linked dyes as well.
If your protein expresses well, you can see it while you growing the culture. Chun Chun Luo, Ph.D. The Protein Expert Accelagen, Inc. 11585 Sorrento Valley Road, Suite 107 San Diego, CA 92121 TeL: 858-350-8085 ext 111 Fax: 858-350-8001 <mailto:[EMAIL PROTECTED]> [EMAIL PROTECTED] www.accelagen.com <http://www.accelagen.com/> _____ From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of deliang Sent: Friday, July 25, 2008 4:26 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] offtopic-"colorful tag" Hi all, I am curious whether there is an expression system in which the target protein will be expressed with a tag, which has a strong extinction coefficient in visible wavelength range? That will dramatically simplify my protein purification and crystallization. Thanks for your suggestion. Deliang
