Dear All, I had initially got some clusters of needles for one of my proteins. I then tried to streak seed them. However, I still seem to get quite a large number of nucleations, hence smaller xtals. I have tried streak seeding in in drops carrying additives such as ethanol, isopropanol, ethylene glycol and MPD (5%), but I now see no growth along the streaks. What are th other things that I could try to improve the quality of crystals?
Thanks in advance. Amit
