Dear Crystallogrphers,
does anybody here know a protocol to get consistently well-diffracting but
smaller, ~50um, cryoprotect(ed/able) lysozyme crystals?
Thanks,
Jacob Keller
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Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [EMAIL PROTECTED]
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----- Original Message -----
From: "Ailong Ke" <[EMAIL PROTECTED]>
To: <CCP4BB@JISCMAIL.AC.UK>
Sent: Thursday, July 03, 2008 4:13 PM
Subject: [ccp4bb] air bubbles in the Bio-Rad Duoflow system
Hello All,
I own a Bio-Rad Duoflow system for almost a year. The machine lived up to
some of the recommendations I saw on this board. However, there is this
annoying problem with air bubbles entering columns and I cannot figure out
the source. The system would be free of any air bubbles in the beginning,
and performs fine when water is loaded using needle/syringe into the 5ml
superloop and then injected into the column. When protein samples are
injected the same way, a lot of air bubbles would appear and get trapped
inside the ion exchange column, leaving yellowish marks on the Uno columns
and eventually reducing their performance. I've been a FLC/AKTA user for
about ten years and have never seen such problems. I doubt it is due to a
faulty injection valve because we recently had it replaced for other
reasons. We did move a backpressure generator (a little black piece) from
post-column position to before-column, otherwise we cannot run sizing
columns in a reasonable flow rate.
I'd like to hear if you have similar experiences and how you fixed the
problem. Thanks a lot!
Ailong
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