Look at the paper (search in pubmed) by B. M. Schick and F. Jurnak on cryoprotection of TuTs. He changes the reservoir in 24 steps using a 24 well cryschem plate. There are some references there that are important too.
Jackie Vitali On Mon, Jun 23, 2008 at 8:14 PM, Ji lee <[EMAIL PROTECTED]> wrote: > Dear, > > I have a crystal diffracted anisotrophically. I tested with a few > different cryo conditions like oil, glycerol in different > concentration to get a better data but these conditions didn't help > any. > Using capillary method improved the diffraction (isotrophic) but the > crystal couldn't survive during the data collection. > Is there any methods or cryo conditions I can use to improve my > diffraction data? > This crystal grew in 2.5M Ammonium Sulfate. > > Thank you so much. > > Ji >