Its not necessary to mask the crosshairs in HKL2000.
This crystal wasn't frozen well. Was it a plate crystal? It looks like
you have some serious problems in your looping. This is an intensely
promising crystal with strong diffraction at lower resolution. Notice
the roundness of the low resolution spots and at the bottom of the
image. If these are plate crystals, you will need to seek out a plate
crystal expert to show you how to loop it.
You have some interesting mosaicity at lower resolution, but I think
this has to do with freezing. If these crystals aren't hard to grow,
you would do better to optimize your freezing conditions than spend a
lot of time trying to extract a structure from this data set. Notice
the 3.2 A reflections even with your freezing and mounting problem.
Don't waste your time processing right now if you can get more
crystals and can get back to your x-ray source. Optimize your freezing
and mounting and the task of solving the structure will be a lot easier.
James
On May 20, 2008, at 12:10 PM, sajid akthar wrote:
Dear All
I have trouble processing my images on HKL2000. Please
refer the attached snapshot of my image.
I dont understand how the cross (hair) lines came into
my images.
Ignoring the lines and continuation of processing
results in high chi square value.
The question is ...
Can I make a mske file to mask all those regions and
do processing. In HKL2000, I believe that I can make
mask file for beam stop and one more region apart from
beam stop. But I do not know whether I can make mask
for all four lines and do processing. If so please let
me know.
Thank you
Sajid
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<imag1.jpg>
--
James Stroud
UCLA-DOE Institute for Genomics and Proteomics
611 Charles E. Young Dr. S.
Los Angeles, CA 90095
http://www.jamesstroud.com
