Try reductive methylation of surface lysines. http://dx.doi.org/10.1016/j.str.2006.09.005
Cheers, Stephen On 5/13/08, Jennifer Han-Chun Tsai <[EMAIL PROTECTED]> wrote: > Hi, > > This topic is not related to CCP4. I am having problem of crystallizing > one protein. It's a pretty small protein with size around 15kDa. I have > stock concentration around 100mg/mL. Crystallization plates I set up are > with concentration of 10mg/mL, 30mg/mL and 50mg/mL. All the plates had been > set up at least one week. Only around 5 wells per plate or less formed > precipitation. The rest of wells are pretty clear still. Is there any > suggestion for reducing protein solubility or increasing the chance of > getting crystals? > > Thanks for your time, > Jennifer -- Dr Stephen Graham Nuffield Medical Fellow Division of Structural Biology Wellcome Trust Centre for Human Genetics Roosevelt Drive Oxford OX3 7BN United Kingdom Phone: +44 1865 287 549
