Dear Oganesyan,
That means the second component is not right based on the number of LLG and PACK. Please see that for more details. http://www-structmed.cimr.cam.ac.uk/phaser/faq.html
You should check that model and redo MR again.
Good luck!
leo
Oganesyan, Vaheh wrote:

Phaser experts,

When running Phaser I see LL gain becoming negative closer to the end. What would that mean?

Resolution ~3.0 A, s.g. I222, 2 complexes per a.u.

Here is the line from log file:

ANNOTATION: RFZ=7.6 TFZ=8.9 PAK=0 LLG=142 RFZ=6.6 TFZ=18.0 PAK=0 LLG=150 RFZ=4.6

   TFZ=12.8 PAK=0 LLG=-44 RFZ=3.8 TFZ=5.8 PAK=19 LLG=-908

Thank you in advance.

///______________________/

/Oganesyan Vaheh, Ph.D/
/Antibody Discovery/
/MedImmune, Inc./




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