Dear All, I want to crystallize a mAb Fab in complex with a hydrophobic cyclic peptide. The peptide contains 1 hydrophilic residue, 2 Cys, and 9 hydrophobic residues. The problem is that I can not dissolve this peptide in common buffers. I think it is need to dissolve it in some organic solvent, such as DMSO. But organic solvent is harmful to protein. Is there any sugestions for this situation?
Thanks. -- Jiamu Du State Key Laboratory of Molecular Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences (CAS)
