Depending on your time, I'd recommend adding the use of a native gel band shift to try a one that doesn't work and one that does.
http://www.doe-mbi.ucla.edu/~sawaya/m230d/Crystallization/crystallization.html I know you can use NaBr or NaI to grow lysozyme crystals as well, and they stick on hydrophobic patches of the enzyme's surface. I do recall, however, that the crystal system changes from tetragonal to the triclinic or monoclinic forms. Bernie On Fri, April 11, 2008 8:09 am, Patrick Loll wrote: > Hi, > > Does anyone have a foolproof recipe for preparing one or more heavy > atom derivatives for a nice, easily crystallized protein like > lysozyme? I'm looking for something that I can use as a hands-on MIR/ > SIR tutorial for beginning students. I'd rather the students be able > to focus their attention on data collection and analysis, rather than > spending a lot of time screening different soaks. > > I have looked at Peter Sun's paper on quick soaking (Acta Cryst D58: > 1092-1098, 2002), and will definitely try what they did; but any > other recipes would also be welcome. > > Thanks, > > Pat > > > ------------------------------------------------------------------------ > --------------- > Patrick J. Loll, Ph. D. > Professor of Biochemistry & Molecular Biology > Director, Biochemistry Graduate Program > Drexel University College of Medicine > Room 10-102 New College Building > 245 N. 15th St., Mailstop 497 > Philadelphia, PA 19102-1192 USA > > (215) 762-7706 > [EMAIL PROTECTED] > >
