Hi, everyone, who has much more experience about the 2-D crystallization of membrane proteins using the dialysis method.
1) how to avoid the change of whole sample's volume after dialysis? 2) can you tell me some tricks during making grids, that is, when we put dialysis sample on grids, your experiences? Thanks in advance, Thanks your kindness; best regards, gxzhao lab of electron cryo-microscopy school of biology Georgia institute of technology Atlanta, GA 30318