Quoting James Stroud <[EMAIL PROTECTED]>:
Try refolding before purification.
OR, you could do a denaturing purification, followed by refolding. Ni-NTA's perfectly viable in denaturing urea, for example. From there, it's just a question of whether to try refolding on-resin, or following elution. Cheers, d.s. =========================================== David M. Shechner, Graduate Student Bartel Laboratory Department of Biology Whitehead Institute for Biomedical Research Massachusetts Institute of Technology [EMAIL PROTECTED] ===========================================
