Dear all, I know most people use glutaraldehyde as the crosslinking reagent for their crystals. But there are a lot of other crosslinking chemicals available both in protein chemistry and histology, such as simple formaldehyde. Did anybody have experience with other chemicals and how did they compared with glutaradehyde? I have a crystal very sensitive to glutaradehyde but I really wants to try the crosslinking method. Thanks,
Nian Huang Dept of Biochemistry UT Southwestern Medical Center Dallas, TX 75390