Have you tried the various oils ?
As a last resort Greek olive oil if paratone and others fail.
How about sugars ?
Stay away from propanol, your crystals will start floating through your
wells with very rapid movements. I once had a 30% 2-propanol crystal
condition and mounting crystals only worked somehow in the cold room and
with lots of luck.
Jürgen
wu donghui wrote:
Dear all,
Recently, I got a crystallization condition as 0.1 M Bis tris ph 6.5,
1.3M lithium sulfate, 0.1M NaCl, the shape of crystals is needle
cluster, very difficult to grow bigger, microseeding does not work,
then I tried macroseeding, and found crystal can grow bigger and rod
like. However as for the cryoprotectant, crystal will dissolve in
glycerol, ethylene glycol, MPD and PEG, even in the very low
concentration about 5%. I found my crystal can grow under the additive
2-propanol in addition to the above mentioned crystallization
condition. I also noticed that Hampton cryoprotectant kit has the
2-propanol as cryoprotectant. I want to know if anyone have ever used
2-propanol as the cryoprotectant and what effect it might be. By the
way Hampton also mention lithium sulfate can be used as
cryoprotectant, the recommended maxium concentration is 2M, I tried
and found ice ring is severe and it is very easily to form lithium
sulfate salt crystal at this high concentration, I also tried sodium
malonate, the diffraction gave me few spots, resolution is only about
6--7 angstrom.
Does anyone encounter such problem by lithium sulfate?
Welcome to any replies. Thanks a lot in advance.
Donghui
--
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002
Web: http://faculty.washington.edu/jbosch
