Dear Crystallographers,
I am looking for a way to modify any specific set of sidechains in my
protein (e.g., all K's, or all D's and E's, etc.). A few requirements:
1. Relatively cheap
2. Definitely non-cell-membrane-crossing
3. Specific
4. Any reasonable amount of change in mass (+ 5-5000 Da?), charge, etc., is
fine
5. Preferably less life threatening to live cells (I know this is a probably
a losing battle)
The last criterion is because I want to modify the protein in vivo, then
immediately harvest the cells, etc. I was hoping there would be something
"around the house" that I could use for this, especially for hitting K's.
All the best,
Jacob
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Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: [EMAIL PROTECTED]
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