Hello, I'd appreciate it if anyone could provide information (experiences or publications) on the following:
1. Put a tag such as FLAG, V5, etc etc, at the N- and/or C-termini, in order for specific detection, but not interfering with protein folding/structure; 2. Is a linker between the tag and target protein needed? What linkers (length, specific sequences) would you suggest? 3. What are the choices of such tags and why would you recommend them? Thank you for your time and sharing in advance. Yongfu Li
