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A
two-year post-doctoral fellowship is available in the “kinetic protein
crystallography” team of the Institute for Structural Biology (IBS), Grenoble, France
(http://www.ibs.fr/content/ibs_eng/presentation/lab/lccp/cinetique/).
The project is financed by the French "Agence Nationale de la
Recherche" (ANR). It consists in studying the fluorescence
mechanisms of fluorescent proteins (FP’s) from the Green Fluorescent
Protein
(GFP) family, by combining X-ray crystallography with UV-visible and
Raman microspectrophotometry.
Fluorescent proteins are typically used as markers in fluorescence cell
imaging. Recently discovered FP’s undergo multiple photo-switching
processes,
which make them invaluable tools to study cell dynamics and develop
super-resolution imaging methods. Such proteins also offer fascinating
perspectives for biotechnological applications such as the development
of
high-capacity storage devices. The work aims at improving fundamental
knowledge
of the photophysics of FP’s, and at rationally designing new FP’s with
enhanced
properties. It will involve both local and international collaborations
with
teams specialized in molecular biology, ab-initio
quantum calculations and cell imaging. The project will be performed in
the
context of a very tight collaboration with the ESRF synchrotron and
will make
intense usage of the Cryobench, a laboratory dedicated to in
crystallo spectroscopic techniques (http://www.esrf.eu/UsersAndScience/Experiments/MX/Cryobench/).
Photo-switching will be triggered in FP crystals following the methods
of
kinetic crystallography, so as to determine the induced conformational
changes
along the switching pathway.
Candidates
should have a strong expertise in crystallography,
biochemistry and UV-visible spectroscopy. A general background in
bio-physics
and an interest for imaging applications is recommended to conduct this
highly
pluri-disciplinary project. Administrative rules state that candidates
should preferably
be less than 30 years and have defended their phD in the last 2 years.
The
monthly gross salary will amount to ~2750 €.
Applications should be sent to Dominique Bourgeois
([EMAIL PROTECTED]).
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Dominique BOURGEOIS
/\ Dominique Bourgeois /\/\
/ \ LCCP IBS /\/ /\
/\/ \ UMR 5075 / \/ \
/ \ \ 41 Rue Jules Horowitz \ \
\ \ 38027 GRENOBLE Cedex 1 \ \
\ \ FRANCE \ \
****** phone: (+33) (0)4 38 78 96 44 ******
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****** e-mail: [EMAIL PROTECTED] ***
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- [ccp4bb] Post-doc position at the IBS, Grenoble, Franc... Dominique BOURGEOIS
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