This has probably been discussed before, so apologies in advance.
We're eyeing a protein that has a probable C4 Zn finger in the
middle. The collaborators who are nicely going to PCR it up want to
know if we'd like it with or without a His tag.
Is it a bad idea to co-mingle Zn-binders and Ni columns? Or is it
likely to bind the column quite nicely without the tag?
thanks,
Phoebe
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Phoebe A. Rice
Assoc. Prof., Dept. of Biochemistry & Molecular Biology
The University of Chicago
phone 773 834 1723
fax 773 702 0439
http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123
http://www.nasa.gov/mission_pages/cassini/multimedia/pia06064.html
