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Hi,
could as well be that your first residue is Gln which frequently cylizes
to pyroglutamate which resists Edman chemistry. In that case you can
deblock your N-terminus with pyroglutamate aminopeptidase (you can get it
from Takara, Sigma, Boehringer, Pierce - whicheverone you like ;-)). THere
are well working standard protocols.
Cheers, Petra
--
PLEASE NOTE THE NEW PHONE NUMBER!!!
Petra Verdino, PhD
Research Associate
The Scripps Research Institute, BCC206
10550 North Torrey Pines Road
CA 92037 La Jolla, USA
tel:1-858-784-2945
fax:1-858-784-2980
