Did you check the pH of your DTT and the protein solution before and after?
I have had some experience with DTT changing pH (as might well be
expected...).
Jacob
*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.467.4049
cel: 773.608.9185
email: [EMAIL PROTECTED]
*******************************************
----- Original Message -----
From: "Joe" <[EMAIL PROTECTED]>
To: <[email protected]>
Sent: Tuesday, November 13, 2007 2:30 PM
Subject: [ccp4bb] DTT sensitive?
Hi there,
I see enormous precipitate of my receptor protein when I take it out
of freezer. But all the precipitate dissolved quickly after I added
1mM DTT to the solution. Does this mean that some surface Cys are
causing problem? Or why is the protein so sensitive to DTT?
Anybody experienced this kind?
Any advice is appreciated.
-Joe
