Quoting Edward Berry <[EMAIL PROTECTED]>: > Savvas Savvides wrote: > > Indeed, but wouldn't consideration of micelle size affect our > > estimation of the number of molecules in the asu, in some cases > > significantly? > Good point- I think now that is taken into account by just saying > "membrane proteins tend to have a high solvent content" and taking > that into consideration when you guess the number of molecules. > But it would be nice to account for the detergent explicitly. > Say by analyzing detergent content of the crystals, or in some > ideal cases neutron diffraction with perdeuterated detergent. >
with regard to this has anyone actually checked how the micelle properties with or without protein "embedded" might differ?? are we assuming empty micelle and the protein-added micelle are the same size/Mw? is this really so? --- of course this may further vary depending on the oligomeric state of the protein --suppose some neutron scattering studies on model systems might give the answer --havent looked. just wondering.. -tommi -- Tommi Kajander, Ph.D. Macromolecular X-ray Crystallography Research Program in Structural Biology and Biophysics Institute of Biotechnology PO box 65 (Street address: Viikinkaari 1, 4th floor) University of Helsinki FIN-00014 Helsinki, Finland Tel. +358-9-191 58903 Fax +358-9-191 59940
