Dear All, I have been trying to get a protein-ligand complex crystal for a long time. Here, ligand is either substrate or product for this kinase. I have tried many methods: soaking with apo-crystal, co-crystal with different concentration of ligand and screen for new conditions. I got a couple of co-crystals with the new conditions, but still no ligand was found in the active site.
Anybody has some new ideas that I can try? I have been using glycerol as my cryoprotectant. Could cryoprotectant have some negative effect on co-crystal? I will try to collect some data at room temperature in the future. Thank you in advance for your suggestion. Nian Dept of Biochemistry UT Southwestern Medical Center
