Is it possible that your crystal is not really a single crystal, or has
multiple domains? I had a similar problem with an otherwise beautifully
diffracting crystal (beyond 2.0 A) whose integration rapidly broke down
after a few frames. On close inspection, it was possible to visualize
multiple lattices in the diffraction images. At some rotation angles,
these were not obvious and indexing was possible over a few frames.
Apparently, my beautiful crystals were in fact stacks of plates slightly
rotated with respect to each other.
Jan Abendroth wrote:
Hi all,
processing data collected on APS beamline BM17 on a MAR165 detector
causes unexpected troubles. Even though the crystals are
well-diffracting and the spots are sharp and well-resolved, indexing
only works with a certain selection of frames, the refinement the goes
totally hairwire. Probalby just a parameter or so set wrong. My input
file in the attachment. The last three lines do not make much of a
difference. Any ideas?
Thanks!
Cheers
Jan
************************
mosflm.in:
detector marccd
directory ../Images
template Image_0###.img
image 001
nullpix 1
separation 0.95 0.95 close
!LIMITS XMIN 0 XMAX 165 YMIN 0 YMAX 165 xscan 2048 yscan 2048
!SIZE 2048 2048
!PIXEL 0.07934
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------------------------------------------------------------------------
Roger S. Rowlett
Professor
Colgate University Presidential Scholar
Department of Chemistry
Colgate University
13 Oak Drive
Hamilton, NY 13346
tel: (315)-228-7245
ofc: (315)-228-7395
fax: (315)-228-7935
email: [EMAIL PROTECTED]
