Rather difficult to decide. It depends on whether BME was present during
all protein purification steps. Also, BME is somewhat short lived. You
have to add new BME every few days or so. At least one looks like it
could be oxidized, a process that cannot be reversed by BME....
J.
Artem Evdokimov wrote:
Hi,
They’re likely both BME adducts, just in the first case the CH2CH2OH
portion is way more disordered.
Artem
------------------------------------------------------------------------
*From:* CCP4 bulletin board [mailto:[EMAIL PROTECTED] *On Behalf
Of [EMAIL PROTECTED]
*Sent:* Monday, August 13, 2007 9:59 PM
*To:* CCP4BB@JISCMAIL.AC.UK
*Subject:* [ccp4bb] extra density on Cysteine
Dear all,
I am refining a 2.0A structure. I found that there were some extra
density on two cysteines, even though I have added 5mM BME in the
protein buffer.
I am wondering whether the first one (Cys292) is a bme and the second
one is an oxidized cysteine. Any suggestion?
I attached the images for your reference. thanks
Regards
_________________________________________
Xu Ting ,Ph.D
10 Biopolis Road
Singapore 138670
Fax: +65 6722 2916
Phone: +65 6722 2980
--
Jeroen Raymundus Mesters, Ph.D.
Institut fuer Biochemie, Universitaet zu Luebeck
Zentrum fuer Medizinische Struktur und Zellbiologie
Ratzeburger Allee 160, D-23538 Luebeck
Tel: +49-451-5004070, Fax: +49-451-5004068
E-mail: [EMAIL PROTECTED]
Http://www.biochem.uni-luebeck.de
Http://www.iobcr.org
Http://www.opticryst.org
--
If you can look into the seeds of time and say
which grain will grow and which will not - speak then to me (Macbeth)
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