There might be many reasons..
Do you have a spacegroup with alternate indexing? If so the two crystals
may not be using the same convention.
See $CHTML/reindexing.html for more details.
Or is one data set a high resolution pass and one a low resolution pass?
Sometimes then there are saturated spots which mess up the scaling..
I usually merge both sets independently first - cad them together and
run SCALEIT to check the degree of agreement.
SCALEIT gives a useful list of outliers and you might be able to
pinpoint the problem from that.
Kianoush Sadre-Bazzaz wrote:
Dear CCP4,
I have a couple of datasets native and heavy-atom-derivatized. The
spacegroup and cell dimensions of all are the same (or very
similar). For some reason I cannot merge any of the datasets
together. If for example I rescale one native dataset by itself, my
Rmerge is about 10%, but when I add 50 images (or so) of another,
supposedly isomorphous crystal, my Rmerge goes up to 20%.
I was wondering if anyone has encountered a similar problem or can
offer any suggestions. Thanks so much,
Kianoush
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