You could try buccaneer to build an initial model, followed by ARP/wARP
to complete the model. I've see some good results on maps in the
2.5-3.5A range with buccaneer, but model completion is still not so good
(although it gets better with each new release).
http://www.ysbl.york.ac.uk/~cowtan/buccaneer/buccaneer.html
Buccaneer is particularly good on well phased maps at lower resolutions
- e.g. after averaging. With high order averaging it should be able to
build at 4.0A.
Tommi Kajander wrote:
Hi,
i would be interested in hearing about people's preferences on programs
for doign auto-tracing of protein chains (with not so great maps), so far
my feeling has been nothing is at least much better than resolve in doing
this. but i was wondering if people would care to share examples on cases
where there was some difference to what you started with...
..of course one can always complete and correct by hand but when you are
doing this with phasing iteratively it would be interesting to hear opinions..
thanks,
tommi
