2007/7/12, Gebhard Schertler <[EMAIL PROTECTED]>:
I have obtained some promising X-tals in 2M Ammonium Nitrate. I am now looking for freezing conditions. Has anybody had experience in successfully freezing X-tals under similar conditions ? What was the best cryo-protectant and which concentration was optimal ?
Hi, You have some informations in supplementary methods of this ref: Structural insight into antibiotic fosfomycin biosynthesis by a mononuclear iron enzyme Luke J. Higgins, Feng Yan, Pinghua Liu, Hung-wen Liu and Catherine L. Drennan Nature 437, 838-844 (1 October 2005) Informations are in the first doc at this page: http://www.nature.com/nature/journal/v437/n7060/suppinfo/nature03924.html 30% xylitol, 2.0M ammonium sulfate, and 100mM Tris-HCl pH 8.0 Some other good cryoprotectants are small PEGs, glycerol, MPD... Michel.
