Quoting Priyank Maindola <[EMAIL PROTECTED]>: > Hello all, > I am struggling with getting the phases out for my protein. Heavyatom > database shows that only mercury and samarium have the binding motifs in > it. > > Sodium iodide soak is killing the crystal even as less as 0.2 Molar > concentration for 30sec. > With Potassium iodide crystal is okay upto 0.75M for 1 & a half min but > the > incorporation is not at relevent sites.(Iodide signal is not distinct and > > is weak)
how much data and what is anomalous redundancy? what space group? could you do Sulphur-SAD? > Hg & Au salts precipitate it. i dont understand this, precipitate the crystals? what concentrations and soak times do you use and what is the pH? ...ie more details would be useful. > With Pt signal is weak. again how about a longer soak??? higher concentration??? ... or try Pd, should be more reactive. > Somewhere I read that high salt causes problems for heavy atom to bind to > > protein.My protein needs high salt for stability (750mM NaCl) I think that would mainyl affect electrostatic binding, but imidazole of cys attachement of Hg, Pr etc probably not. (??????) and again, why no se-met?????? > Please help with some suggestion!! > tommi
