This notice is posted at the request of Wim Hol. Interested candidates should
direct their inquiries to him at the address below.
Thanks
Stewart
RESEARCH SCIENTIST POSITION AVAILABLE
Molecular Biology for Structural Studies on Proteins from Global Pathogens
JOB DESCRIPTION:
The projects in the protein crystallography group of Wim Hol are all
focused on providing a basis for development of new therapeutics to combat
tropical diseases. Current projects include molecular machines, protein-RNA
complexes and integral membrane proteins. In particular the sophisticated Type
2 Protein Secretion system of cholera toxin, the unique RNA editing machinery
in mitochondria of the sleeping sickness parasite, and the essential malaria
parasite invasion machinery are at the center of attention.
The responsibilities of the successful candidate include:
(i) Design and construct plasmids and multi-plasmid systems to express and co-express protein of interest in E. coli, and in other expression systems like insect cells;
(ii) Carry out site directed mutagenesis to obtain variants of the proteins
under investigation with respect to length, active site characteristics,
surface properties, etc.;
(iii) Small scale expression of proteins and multi-protein complexes;
(iv) Prepare large quantities of RNA by in vitro translation methods;
(v) Maintain molecular biology, protein expression and protein purification
equipment;
(vi) Maintain the plasmid and expression system database of the group;
Further information regarding the work of the laboratory can be found at:
www.bmsc.washington.edu/WimHol
depts.washington.edu/biowww/faculty/hol.html
JOB REQUIREMENTS:
- At least three years of experience with:
- Constructing vectors with optimal properties for producing protein with
various cleavable and non-cleavable affinity-chromatography purification tags.
- Site-directed mutagenesis;
- Over-expression of eukaryotic proteins in E. coli or other heterologous
expression systems;
- Management of databases containing plasmid, vector and expression information.
- Maintaining molecular biology equipment.
- Molecular biology software and web-based programs.
- Ability to work on multiple projects simultaneously with minimal supervision.
- Excellent interpersonal skills to function optimally in the project teams and
to cooperate with collaborators in other institutions.
- B. Sc. in biochemistry or microbiology, or equivalent.
DESIRED SKILLS (not required):
- Experience with production of large quantities of RNA by in vitro translation
methods; and/or protein expression using baculo virus and insect cells; and/or
application of phage display techniques.
Interested candidates are invited to send an application plus brief CV plus
names and email addresses of three references to: Wim Hol at [EMAIL PROTECTED]
