Good day all, I have what may be a very simple question. I am trying to insert a substrate/ligand into the active site of my enzyme using COOT, into electron density that I have already utilized in "O" for this purpose. I've created the substrate library file (*.cif) using a pdb file from PRODRG in the ccp4 sketcher. In COOT, I went to File->Get Monomer..., but when I type the 3 letter code, I get nothing.
Further, I have imported the substrate as a separate pdb file, and can move it close to the active site, but I have no idea how to orient/manipulate the ligand into the electron density. If I can eventually get the ligand as a monomer into the screen, I still don't know how to manipulate it's orientation prior to writing it into the enzyme's pdb, so I guess that I'm just generally stuck here. Any help/suggestions/advice would be appreciated. Thank you all for your time here. ____________________________________________________________________________________ Need Mail bonding? Go to the Yahoo! Mail Q&A for great tips from Yahoo! Answers users. http://answers.yahoo.com/dir/?link=list&sid=396546091
