Hello,
I've got a protein that forms oil phase in almost all conditions in the
screen. No xtals thus far. I was wondering if there are general hints on
dealing with such cases. Basically, I am trying to understand what
direction to go from here. E.g. decrease or increase [protein], [salt] and
general precipitant strength? High concentration of some solvent
everywhere? Detergents?
The protein is small, forms dimer via short coiled coils region and and
various prediction servers say that the rest is likely to be disordered/not
globular. In low to moderate salt without precipitants it behaves well -
single peak on gel-filtration, does not aggregate, can be concentrated to
at least 30 mg/ml.
Thanks,
Dima
