Dear friends, Recently, I have solved a structure using mad method. When using the peak data(2.3A) as the native for structure refinement, the gap between R factor and R free is big, about 0.1(0.22 and 0.32). I modelled the selenomethionine but the gap still exists. When I changed the data for a real native one(2.7A),it seems OK with R=0.24 and Rf=0.28.
Does anyone have the similar experience? what should I pay attention to when using the sad/mad data as the native one for modelling and refinement? Thanks in advance. -- Peng Zhang, Ph.D. Student Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences 320 Yue-Yang Road Shanghai 20031 P.R. China Tel: 021-5492-1117 Fax: 021-5492-1116 Email: [EMAIL PROTECTED]
